Objective To investigate the efficacy of three mosquito attractants (Attractant A, B, and C), carbon dioxide (CO ₂) at different flow rates and attractant combined with CO ₂in attracting Aedes albopictus, to screen out the optimal combination, and to provide a scientific basis for the application of chemical pheromone monitor in the field work of Ae. albopictus surveillance. Methods A remote-controlled chemical pheromone mosquito trap monitor was used to perform the trapping test. A total of 200 Ae. albopictus mosquitoes were placed in the simulation room, and the trapping test was conducted for the three mosquito attractants, CO ₂ at different flow rates, and attractant combined with CO ₂, and efficacy was observed after 24 hours. The Chi-square test and the logistic regression analysis were used to compare and analyze the efficacy of different attractants and CO ₂ flow rates in trapping Ae. albopictus. Results CO ₂ at a flow rate of 80, 150, and 200 ml/min had mean trapping rates of 50.42%, 64.67%, and 74.33%, respectively, and attractant A, B, and C had mean trapping rates of 66.40%, 70.30%, and 53.00%, respectively. Attractant A combined with CO ₂ at a flow rate of 80, 150, and 200 ml/min had mean trapping rates of 66.67%, 67.33%, and 79.67%, respectively, and attractant B combined with CO ₂ at a flow rate of 80, 150, and 200 ml/min had mean trapping rates of 70.67%, 78.67%, and 82.33%, respectively. Conclusion In the simulation test, the remote-controlled chemical pheromone mosquito trap monitor with the application of mosquito attractant and CO ₂ has a marked effect in trapping Ae. albopictus, which is better than the use of attractant or CO ₂ alone. The surveillance method can be used for field surveillance and control of vector Aedes mosquitoes.

Objective To investigate the interspecific competition between Aedes aegypti from Wushi town of Leizhou and Ae. albopictus from different places in Guangdong province, China, and to evaluate the dispersal ability of Ae. aegypti. Methods From March to August 2017, 100 second-instar larvae of Ae. aegypti from Wushi town and 100 second-instar larvae of Ae. albopictus from one of selected places in Guangdong province were mixed and raised in a mosquito room. Eighteen days after emergence, all adult mosquitoes were collected with an electric mosquito vacuum for classification and counting, and all mosquito eggs were collected and put in water for next-generation rearing. A total of six generations of mixed rearing were conducted to compare the competitiveness of different species by the number. Results In each generation, Ae. aegypti was the first to emerge, and Ae. albopictus emerged more than three days later. The number of Ae. aegypti from Wushi town of Leizhou was more than the average number of Ae. albopictus in this town and the number of Ae. albopictus from Meizhou in each generation; there were five generations more than that from Maoming, one generation less; there were four generations more than that from Qishui town of Leizhou, Shaoguan and Shenzhen, two generations less; there were two generations more than that from Zhanjiang, four generations less; there was one generation more than that from Guangzhou, five generations less. Conclusion Aedes aegypti from Wushi town of Leizhou has strong competitiveness against Ae. albopictus from Meizhou, Maoming, Shaoguan, and Shenzhen of Guangdong province.

Objective To investigate the mosquito infestation in the mouths of manholes under the municipal administration networks in Shenzhen, Guangdong province, China, and to provide a basis for the prevention and control of mosquito-borne diseases in urban areas. Methods The ponding status, mosquito infestation rate, and mosquito density were investigated in the mouths of manholes under the municipal administration networks of representative subdistricts in the new town areas, old town areas, and urban villages in Shenzhen. The larvae were captured with a 500-ml larva sampling spoon for density (larvae/spoon) calculation. Results A total of 508 mouths of manholes under the municipal administration networks were investigated in November 2017; the mean positive rate of mosquito infestation was 4.7% (including a mean positive rate of Aedes infestation of 3.3%). The mounting rate and maintenance rate of Mosquito Proof Set (the four types of mosquito proof facilities, including mosquito repellent sticks, mosquito proof sluices and nets) were 27.4% and 76.3%, respectively. Of the mouths of positive manholes, 83.3% were not equipped with Mosquito Proof Set. The highest and lowest densities of larvae in the positive manholes were 43 larvae/spoon and 1 larva/spoon, respectively. Relatively high positive rates were observed in water supply manholes (5.9%), sediment manholes (5.7%), and municipal sewage manholes (5.5%). The sediment manholes, municipal sewage manholes, and municipal rainwater manholes had a relatively high constituent ratio both in the overall manholes investigated (84.8%) and in the positive manholes (87.5%). No mosquito infestation was found in the mouths of gas, communication, and power supply manholes. Conclusion The Mosquito Proof Sets are conducive to reducing mosquito infestation in the sewers. Places most seriously infested with mosquitoes are sediment manholes, municipal sewage manholes, and municipal rainwater manholes under the municipal administration networks. The Mosquito Proof Sets can effectively reduce mosquito infestation.

Objective To understand the seasonal variation in the population density of Aedes albopictus in Guangdong province, China, based on a standard surveillance method, and to provide a scientific basis for the early warning and prediction for Aedes-borne diseases. Methods From 2007 to 2017, the population density of Ae. albopictus in Guangdong province was monitored by Breteau index (BI), mosquito ovi trap index (MOI), and the mosquito light trap method. Results The Ae. albopictus larvae density surveillance in 9 506 102 houses resulted in a mean BI value of 2.94. The adult Ae. albopictus density surveillance using 1 428 078 effective mosquito ovitraps resulted in a mean MOI value of 4.98. The adult Ae. albopictus density surveillance using 82 019 effective mosquito light traps resulted in an overall Ae. albopictus density of 0.21 mosquito/lamp·night. Between-group comparison made by analysis of variance and the Bonferroni method showed that there was a significant difference in BI surveillance results between different environments ( F=69.158, P<0.01). However, there were no significant differences in MOI or mosquito light trap surveillance results between different environments ( F=1.642, P=0.174; F=1.973, P=0.081). According to the surveillance results by the three methods, the population density of Ae. albopictus was higher in summer and autumn than in winter and spring. Conclusion Aedes albopictus exists all the time of a year in Guangdong province. The population density of Ae. albopictus varies with year, and season. Therefore, the prevention and control of Ae. albopictus should be performed on a case-by-case basis. The sensitivity of a surveillance method varies with environment, suggesting that the selection of surveillance methods should depend on the surveillance environment.

Objective To understand the distribution status of Aedes aegypti and Ae. albopictus in Leizhou and assess the risk of dengue and Zika virus diseases transmission. Methods From July to August in 2017, using remote sensing technology and geographic information system (GIS), the distributions of Ae. aegypti in Wushi, Qishui and coastal villages of Leizhou, Guangdong province were investigated by larval straw method and mosq-ovitrap method. Results Seventy-nine (79) residential buildings and 23 fishing vessels were investigated in Wushi and 55 residential buildings and 28 fishing vessels in Qishui. There were 34 positive places of Aedes mosquitoes in Wushi, of which 17 were positive for Ae. aegypti, 14 were positive for Ae. albopictus, and 3 were positive for both. There were 17 positive places of Aedes mosquitoes in Qishui, of which 4 were positive for Ae. aegypti and 13 were positive for Ae. albopictus. In total, 308 bodies of water in 15 coastal villages from Liusha to Jijia were investigated and only Ae. albopictus was found without Ae. aegypti. Aedes aegypti mostly breed in water storage containers of residential buildings and fishing vessels, while Ae. albopictus mostly breed in outdoor tires. Conclusion Aedes aegypti was only found in Wushi and Qishui in Leizhou, and its population and distribution range had a decreasing trend. Large-scale wooden fishing vessels in the two towns still had the Ae. aegypti distribution.

Objective To assess the mosquito-killing effect of ultra-low-volume spraying machine during night and day in community and Zhongshan Park at Shaoguan, and provide the scientific basis to choose scientific and effective mosquito-killing technology. Methods Used the large vehicle-mounted ultra-low-volume machine spraying pesticide to kill mosquitoes in Shaoguan communities and park environment, respectively during night and day, then evaluated mosquito-killing effect of applying pesticide spray during night and day by Mosq-ovitrap positive index (MOI) and human-bait of monitoring Aedes density before and after applying pesticide. Results After ultra-low-volume spraying during night and day respectively at villa of Shaoguan Country Garden, human-bait index was reduced by 98.19% and 76.85%, MOI by 81.15% and 43.86%; at park, human-bait fell by 76.47% and 77.78% respectively, and MOI fell by -58.54% and 76.33% respectively. Human-bait relative density index of nighttime, daytime spraying at villa was 3.90 and 14.25 respectively, MOI was 38.48 and 24.47; human-bait relative density index at park was 33.82 and 44.44, MOI was 99.09 and 45.68. Conclusion The ultra-low-volume spraying in Shaoguan community environment at nighttime was significantly better than daytime spraying, but in Zhongshan Park the results were similar. In community environment, nighttime ultra-low-volume spraying, compared with spraying during the day, can effectively kill mosquitoes and keep mosquito-borne diseases at low density level, and has a certain application prospect.

Objective To study the efficacy for mosquito control and residual effect of different pesticides by artificial simulation as barrier spray on vegetation. Methods Using insecticidal barrier spray on vegetation technology, Scheffleraoctophylla were sprayed separately by 2.5% effective chloric fluorine with ester millirod suspending agent (Demand), 10% alpha-cypermethrin (Fendona) and 10.4% chlorine chrysanthemum-allyl chrysanthemum water emulsion (alpha-cypermethrin). Then they were moved to an outdoor tent. A total of 30 female Aedes albopictus were put into every tent weekly. One rat and four mosquito oviposition device were introduced into every tent after 24 h, then collected mosquito eggs after 3 d, and recorded survived mosquitoes. Results 2.5% effective chloric fluorine with ester millirod suspending agent remained effective for more than 21 weeks, 10% alpha-cypermethrin for 9 weeks, and 10.4% chlorine chrysanthemum-allyl chrysanthemum water emulsion had no residual effect. The maximum of weekly counts for survivors was 4, 15 and 21 respectively. The median was 1, 5 and 7. Conclusion Both 2.5% effective chloric fluorine with ester millirod suspending agent and 10% alpha-cypermethrin have good mosquito killing and residual effect by using insecticidal barrier spray on vegetation, 2.5% effective chloric fluorine with ester millirod even better.

Objective Application of insecticidal barrier spray on vegetation for dengue mosquito vector control to explore rapid and long-term effective control means for dengue mosquito vector control in the urban communities. Methods From June to September of 2015, the phoenix garden of Shaoguan city in Guangdong province was selected as the test site, comparing the effectiveness of barrier spray on vegetation technology to kill the dengue vector mosquito with the ultra low volume spraying. Results The insecticidal barrier spray on vegetation showed quick kill effect, and continuous control effect on Aedes albopictus, reducing mosquito lure egg apparatus index (MOI) lure under 5 for 2 to 4 weeks, the duration of the MOI under 10 for 3 to 6 weeks, the duration of the effective control of mosquito density for 6 to 7 weeks. Pesticide resistance was not detected. Conclusion Insecticidal barrier spray on vegetation can effectively control dengue mosquito vector density more than one month. Compared with ultra-low volume spraying, insecticidal barrier spray on vegetation reduced the cost by 37.50%, saved on-site manpower, pesticides and time, had a positive impact on dengue fever outbreak control in urban communities.

Objective To investigate the resistance status of Aedes albopictus to used insecticides in the late stage of dengue fever emergency control of Guangdong province in 2014, and learn how to prevent the development of resistance. Methods From the end of October to the beginning of November 2014, larve of Ae. albopictus were collected in epidemic regions in Haizhu and Baiyun district of Guangzhou city, Chancheng and Nanhai district of Foshan city, Chaoyang district of Shantou city, Chao'an district of Chaozhou and Rongcheng district of Jieyang city, and were reared about 1 to 2 generations in the lab, then were tested along with the susceptible larvae according to the methods of national standard “Test Methods of mosquito resistance to insecticides-bioassay methods”(GB/T 26347-2010) to calculate LC₅₀ and resistance index. Results Aedes albopictus of all tested regions had low resistance to temephos and propoxur, the resistant index was 4.92-8.33 and 2.35-6.23 fold respectively. The resistantce to permethrin, beta cypermethrin, deltamethrin resistance were 3.53-31.29 fold, 8.35-38.69 fold, and 2.63-37.04 fold respectively. Of pyrethroid insecticides, Guangzhou city have reached the high level resistance mostly, Foshan city, Chaozhou city mostly low level of resistance, Jieyang city for low level of resistance, Shantou city mostly above the moderate level of resistance against cypermethrin. Aedes albopictus from some cities also have developed resistance to other insecticides in different levels. Conclusion Resistance of Ae. albopictus in the dengue epidemic cities in Guangdong increased very quick to the used insecticides. The control of Ae. albopictus should adhere to the principle of sustainable and integrated management to prevent or delay the development of resistance.

Objective To investigate the resistance levels of Musca domestica to four commonly used insecticides in the special places such as landfill and meat processing plant in Guangdong province, China. Methods M. domestica larvae were collected in the wild breeding habitats, and topical application was used to determine the median lethal doses (LD₅₀) of permethrin, deltamethrin, beta-cypermethrin, and DDVP for M. domestica. The experimental data were statistically analyzed by extremum method. Results The LD50 of permethrin, deltamethrin, beta-cypermethrin, and DDVP were 13.7179, 3.5971, 5.5982, and 0.7704 μg/insect, respectively, for M. domestica in the landfill and were 1.0999, 0.7278, 8.3314, and 0.5322 μg/insect, respectively, for M. domestica in the meat processing plant. The resistance ratios were 58-2525, increased 1-103 times compared with those in 1996 and increased 2-1383 times compared with those in 1991. The resistance of M. domestica to permethrin, deltamethrin, and DDVP was higher in the landfill than in the meat processing plant. Conclusion M. domestica develops high resistance to some insecticides in the special places in Guangdong province, and the resistance to pyrethroid increases rapidly. Comprehensive management strategies and proper insecticide use should be adopted to control M. domestica in the special places such as landfill and meat processing plant.

Objective To determine the characteristics and dynamics of mosquito population during the process of rapid urbanization, and to identify the relationships between the dynamic of main vectors and rapid urbanization. Methods The mosquito density was investigated for the past four years using the human-bait method. Results Five species of mosquito were captured, including Culex tritaeniorhynchus, Culex pipiens quinquefasciatus, Anopheles sinensis, Aedes albopictus, and Armigeres subalbatus. Cx. pipiens quinquefasciatus was predominant (92.27%), followed by Ar. subalbatus (3.98%). The proportions of Ae. albopictus and Cx. tritaeniorhynchus were relatively small, accounting for 1.62% each. The smallest was An. sinensis (0.51%). During a four-year study period, significant changes had taken place in the proportions of mosquito populations by comparison among the data in 2006, 2008 and 2009. The population of Cx. pipiens quinquefasciatus decreased while increased of that of Ae. albopictus. An. sinensis gradually disappeared as the time elapsed. The poplation of Cx. tritaeniorhynchus fluctuated largely in the study periods. In the evening, varieties of mosquito species showed distinct activity patterns. The biting time of Cx. pipiens quinquefasciatus and Cx. tritaeniorhynchus was from 19: 00 to 22: 00 in the evening with a peak after 21: 00. Ae. albopictus could be caught all the night with the main peak before 20: 00. A similar activity pattern was observed from Ar. subalbatus and Ae. albopictus. Conclusion During the process of rapid urbanization, the population of the dengue vector, Ae. albopictus, increased gradually. The population of the Japanese encephalitis vector, Cx. tritaeniorhynchus, fluctuated greatly in different years. However, the malaria vector, An. sinensis, eventually was not found. Thus, we propose that the surveillance and control of Ae. albopictus and Cx. tritaeniorhynchus should be strengthened during the process of urbanization.

【Abstract】 Objective To evaluate the attractant efficiency of Mosq-ovitrap combined with attractants to mosquito in the laboratory and field. Methods The study was carried out in laboratory and field in Guangzhou from September to November in 2007. The Mosq-ovitrap in tested group with yeast-generated CO₂, Panicum maximum infusion, tyre infusion, L-lactic acid dilution, or darken surface and that in control group with tap water were set at intervals of 20 cm, respectively. Mosquitoes captured and the egg positive traps were counted and recorded every day. Results Comparing with the control group, yeast-generated CO₂ could take a strong attraction to mosquitoes in the field (P＜0.05), but had an adverse effect in laboratory. P. maximum infusion, tyre infusion and L-lactic acid dilution could not attract Aedes albopictus to oviposit in Mosq-ovitrap. The colour change of Mosq-ovitrap could not raise its attractant ability to mosquitoes, on the contrary, increase the probability of mosquito escaping from Mosq-ovitrap. Conclusion Yeast generated CO₂ as attractant could significantly raise the attractant efficiency of Mosq-ovitrap in underground parking. However, that of P. maximum infusion, tyre infusion, L-lactic acid dilution and colour change of Mosq-ovitrap was not obvious.

【Abstract】 Objective To test the efficacy of four kinds of electric liquid repellents (A 0.12% dimefluthrin, B 0.17% dimefluthrin, C 0.86% transfluthrin, D 0.86% prallethrin) for the Japanese encephalitis(JE) vectors in the field. Methods The landing count method was used to the investigation of mosquito density before and after treatment by electric liquid repellent. Results The main species caught in the field were Culex pipiens quinquefasciatus and Cx. tritaeniorhynchus, which were the JE vector. Only  sample  A  reached ＞80%  population  reduction  of  Cx. tritaeniorhynchus. Both sample B, C and D caused ＜80% population reduction of Cx. tritaeniorhynchus. Both sample A, C and D caused ＞80% population reduction. However, only sample B led to 70%-80% population reduction. Conclusion The tests suggest that four samples have a good repellent effect on mosquito, but the efficacy of sample B is less than that of sample A, C and D. There are different for the control efficacy of different electric liquid repellent in the lab and the field.

【Abstract】 Objective To study the resistance of Rattus rattus sladeni to anticoagulant rodenticide. Methods No?choice food test was used in this study according to the method of national rodent resistance surveillance group. Results The rodents caught in nine districts of Guangzhou were tested. The survival rate  was 3.8%. The total average dosage was 30.31 mg/kg, and survival  average dosage  was  58.29 mg/kg.  The  result  showed  that  its  sensitivity  to  anticoagulant   rodenticide  was  between R.tanezumi and  R.norvegicus.  Conclusion The  anticoagulant  rodenticide can be still used to the control of R.rattus sladeni. But it should strengthen the resistance surveillance, and the chemicals should be used scientifically and rationally. 

【Abstract】 Objective To develop a system of stepwise response for risk categories for dengue fever in China. Methods According to ecological features of Aedes albopictus, establish the method of mosquito and oviposition trap and compare the relationship of the mosquito and oviposition positive index （MOI） and the Breteau index （BI） or the Ovitrap index obtained from the traditional dengue fever vectors surveillance. Develop an epidemic forecast and phased response system for dengue fever control and prevention with reference to other correlative grade control system for dengue or other vector?borne disease, combined with the actual application of mosquito and oviposition trap in the vector surveillance. Results The MOI could be classified into 4 levels, below 5, 5-20, 20-40, and ＞40 respectively. Stepwise response for risk categories for dengue fever were divided into 4 levels according to the levels of MOI, seasonal character, virus infection rates in mosquitoes and the result of human cases surveillance. Conclusion The MOI is less than 5, which suggestes the control of dengue fever is very effective. However, the risk categories and its response index still need further study and verify in the practical application.

【Abstract】 Objective A field study was carried out in a restaurant in the south of China to investigate the blood sources affected Japanese encephalitis （JE） vectors Culex tritaeniorhynchus and Cx.pipiens quinquefasciatus indoor abundance. Methods Vector density was investigated by human?leg capture technique. Results In the dinning room adjacent to deer blood resource, Cx.tritaeniorhynchus was the dominant specie（74.80% of total number） and the minor vector was Cx.pipiens quinquefasciatus （21.14%）. However, in the restroom where the mosquitoes could only access to human blood,  Cx.pipiens quinquefasciatus accounted for 60.28% and that of Cx.tritaeniorhynchus was 36.62%（n＝845）. The density of Cx.tritaeniorhynchus in restroom was higher than that in the dinning room （df＝1, χ2＝5.32，P＜0.05, n＝676）. However, the density of Cx.pipiens quinquefasciatus in the dinning room was higher than that in the restroom（df＝1, χ2＝268.45， P＜0.001, n＝611）. Conclusion The results showed that the blood resources influenced the JE vector spatial abundance. The abundance of Cx.tritaeniorhynchus or Cx.pipiens quinquefasciatus was affected by the animal blood or the human blood.

Objective To study the resistant change of Xenopsylla cheopis to insecticides in plague natural focus. Methods Surface contact method was used in this study. Results The LC ₅₀ of X.cheopis to malathion, propoxur, dichlorovos, permethrin, deltamethrin and fendona in the constant flea-killing areas were 0.0165, 0.1170, 0.0202, 0.0002, 0.0038, 0.0087 mg/cm ², which were 4.78, 16.32, 2.08, 1.70, 1.66 and 2.94 times of the control. Conclusion Large and constant use of flea-insecticide may lead to resistance. Therefore, the resistance of insecticide must be monitored constantly when killing fleas. The insecticide must be used scientifically and rationally.

Objective To investigate the breeding characteristics of Aedes albopictus in urbanization residential area,which should provide the basis for the control of Ae.albopictus. Methods The study was carried out in the village of Jieyang city and high-urbanization downtown of Foshan from March 2004 to November 2005. Water containers were detected in and around one household which was randomly selected every 5 households. The data were analyzed by software SPSS 11.0. Results Of 5635 possible breeding sites in 7470 houses investigated, 502(6.72%) were positive. In the village of Jieyang,the major breeding containers included unused container (jars, bottles and tins, etc.) and water reservoir receptacles with the positive constitute ratio of 64.98% and 18.77% respectively. The positive constitute ratio of unused containers reached the maximum in September every year. But in residential area of Foshan city, the major breeding containers were made up of water vases in which the flowers and plants grow and unused containera, and the positive constitute ratio of them were 45.52% and 33.79% respectively. The positive constitute ratio of unused containers was in the peak from July to September, and that in miniascape and water plant was the highest in 2004 and May 2005 with the value of 75.00%. But the result was zero after May 2005. Conclusion There were different among the breeding sites in different residential area, which varied with season. The breeding containers in increasing urbanization cities were mainly miniascape, water plant and unused containers, but in low urbanization village, the breeding sites were mainly the unused containers and water reservoir receptacle.

Objective To study the insecticide resistance development of in Xenopsylla cheopis after insecticide using for several constantly years. Methods Bioassay of flea contact insecticide effective testing membrane was used to carry out the surveillance. Field population in the insecticide applying areas for plague vectors prevention and control fleas was collected and tested, the flea population from non-insecticide using areas as control. Results Each LC _{50 of X.cheopis to malathion, propoxur, dichlorovos, permethrin, deltamethrin and FENDONA in the constant flea-killing areas was 20.20, 3.75, 0.17, 16.50, 117.00 and 8.65 μg·ai/cm² which was 2.08, 1.66, 1.70, 4.78, 16.32 and 2.94 times higher than that of the un-insecticide applying contrast areas. Conclusion Applying insecticide constantly in plague vector fleas control may lead to flea insecticide resistance. It’s better to know the insecticide susceptibility background when we develop a strategy for plague flea vectors prevention and control.}

Objective To study the sensitive of TaqMan MGB Real-time polymerase chain reaction(PCR) and nested RT-PCR on detecting Dengue virus(DV) from Aedes albopictus and establish a sensitive, specific, and repetitive method for DV diagnosis. Methods Females adults were artificially infected with Den-2 in lab, different infected mosquito concentration (mosquitos/1000 μl) and 50 mosquitos/pool were designed and processed for virus detection by TaqMan MGB Real-time PCR and nested RT-PCR. Fluorescence signal and electrophoresis showed the results. Results For all pool, the lightest concentration that can be detected was 3 mosquitos/1000 μl for two-step TaqMan MGB Real-time PCR, and 5 mosquitos/1000 μl for one-step TaqMan MGB Real-time PCR and nested RT-PCR. Conclusion Two-step TaqMan MGB is more sensitive, specific, scientific and rapid to detect DV than nested RT-PCR, and is a good surveillance method for DV in A.albopictus.

Objective In order to test the efficacy of four kinds of mosquito coils in the field,and to investigate the efficacy tests and evaluation methods for different kinds of mosquito coils.Methods Compare with density reduction by the bite counts based on pre-and post-treatment using human-leg catch technique.Results The main species which was caught in the trial field was Culex pipiens quinquefasciatus and decreased the peak of bites period was from 22:00 to 23:00 in the evening.Coil A and D caused 88.2%,90.5% population,respectively.However,coil B and C led 72.5%,78.1% population reduction,respectively.Conclusion The tests suggested that four coils had a good effect on prevention from the bites of the mosquito,yet,the insecticide efficacies of coil A and D are better than that of coil B and C.Discussion: Pre-trial is very essential for the efficacy tests in the field trial.Simulating field test for indoor insecticidal efficacy should be adopted to correct or reduce the experiment error.

Objective To study the bite rate,epidemiological a village,named T,in Guangdong province distribution,clinical symptoms of the villagers bitten by the red imported fire ants (RIFA) and the environment of to provide evidence for disease control and prevention. Methods Using random sampling method to spot-check households in the village and interview with questionnaires to profile the epidemiological characteristics,clinical manifestations and the treatment of the T villagers bitten by the RIFA. Results During the period of January 1 to June 28,2006,among 60 households surveyed,45 households were bitten by the RIFA. The household bite rate was 75.0%. In the total of 241 people questioned,72 people had been bitten by the RIFA. The bite rate of population was 29.9%(72/241). Among those afflicted people,there was no significant difference between male and female; for villagers more than 40 years old,the bite rate was higher than 50%. In the extremely hot June,the number of villagers bitten by RIFA drastically increased. Workers at farm and/or afforesting belts were much more likely to be bitten by RIFA. The bite rates in farmers and afforesting workers were 42.3% and 28.3% respectively. The proportion of bitten feet was the highest as 65.0%,the next was the hand; The clinical manifestation were mainly including itch- pain(100%),blushes(45.8%),skin rash(78.6%),papule(54.2%),water(pus) blister(33.3%),fever rarely(1.4%) and dizziness(1.4%) and so on 98.6% patients of which were treated by themselves. The nest of RIFA scattered in the village. Conclusion The villagers bitten by RIFA is common in the village in the recent years but with less severe clinical manifestations. We should enhance the health education for the farmer and afforesting workers,and enhance to control RIFA by the scienific ways.

Objective To compare the results and understand relationship between the mosq-ovitrap method and larval survey for detecting the presence of ; Aedes mosquitoes in the field. Methods The study was carried out in the cities of Foshan and Jieyang,Guangdong province,from March 2004 to November 2005. A mosq-ovitrap was set every 5 random households and their premises,or set every 25-50 meters in a hidden and shady place in the surroundings of hospitals,schools,government office buildings,factories and gardens. Larval surveys and mosq-ovitrap method were used simultaneously in the households or in the areas of 5 meters around the trap set in the surroundings. Results In households,hospitals,schools,government office buildings,factories and gardens,14 902 mosq-ovitraps were simultaneously set and 14 648 were collected back. The positive traps for adult mosquitoes was 932. The mosquito and oviposition positive index(MOI) and mosquito-trap density index(MDI) were 6.36 and 1.03± 0.89 respectively. Total 962 Aedes albopictus(99.5)and 5 Culex(0.5) were collected. The maximum of the mosquito adults captured in a single trap was 10. At the same time,8537 water containers in 14 902 households or premises (5 meters in circumference of the mosq-ovitrap) were checked,of which 844 contained the Aedes albopictus larvae. The Breteau Index,House Index and Container Index were 5.66 ,3.87 and 9.89 respectively. The MOI was 1.12 times of the Breteau Index,or 1.64 times of the House Index,or 0.64 times of the Container Index. Conclusion There is a positive relationship between the MOI and the Breteau Index,and the House Index,the container Index respectively. The MOI is particularly approximate to the Breteau Index.

Objective To compare the results between the mosq-ovitrap and the Ovitrap in the field. Methods The trial was carried out in a campus in Guangzhou from August to November 2004,and from February to August 2005. One mosq-ovitraps and one ovitraps at intervals of 1 m were set every 50 m at hidden and shady spot and the positions of two kinds traps were alternated each month. Mosquitoes captured and the egg positive traps were counted and recorded on the 4 ^th day and the 7 ^th day since the installation. Results On the 4 ^th day since installation,the positive indices of mosq-ovitrap and ovitrap were 35.5 and 56.7 respectively; and at 7 ^th days,the positive indices were 53.3 and 71.5 respectively. In total,the oviposition index(OI) of the ovitraps at 7 ^th days was nearly 2 times of the mosquito and oviposition positive index(MOI) of mosq-ovitraps installed for 4 days. 284( 99.3 ) Aedes albopictus,and 2 Culex quinquefasciatus were captured in the 662 mosq-ovitraps installed for 4 days . The mosquito(Aedes)-trap density index(MDI) was 1.21± 1.12 ,the number of caught mosquitoes ranged from 0 to 8 in all the mosq-ovitraps. The results of the surveillance in each month indicated that there was positive correlation between the MOIs of the mosq-ovitraps set for 4 days and the OIs of the ovitraps set for 7 days ( r=0.838 , P=0.001, y=25.548+1.312 x). Conclusion When the mosq-ovitraps were applied for 4 days,the MOI could reflect the population seasonal dynamic of mosquito. There was positive correlation between the MOIs of the mosq-ovitraps and the OIs of the ovitraps.

Objective To study the resistance development of anticoagulant rodenticide in commensal rodents. Methods Tests was carried out without selectivity by rodents based on the method of National Rodenticide Resistance Monitoring Co-worker Group. Results 769 Rattus flavipectus and 303 Rattus norvegicus were caught in five cities of Guangdong were tested. The resistance rate differs from 1.69 to 12.21 the average dosage taken differs from 10.36 mg/kg to 131.96 mg/kg . The result showed that Rattus flavipectus and Rattus norvegicus had become individual resistance to the resistance rodenticide Ⅰ. Conclusion The anticoagulant rodenticide Ⅱ should be used in the areas gathered with Rattus flavipectus. The anticoagulant rodenticide Ⅰ can be still used but should be used scientifically and properly with careful tests of sensitivity.

Objective To survey the resistance status of Aedes albopictus to several commonly used insecticides in Guangdong province,and learn how to prevent the occurrence and development of resistance. Methods Larvae of Ae.albopictus were collected in five cities located in the east,south,west,north and middle of Guangdong province,and were bred for 1 to 2 generations in the lab,then tested with WHO standard bioassay to calculate LC ₅₀ and resistance index. Results Ae.albopictus had low resistance to deltamethrin and cypermethrin,the resistant index was 3.10-4.20 against deltamethrin,and 2.23-2.91 against cypermethrin. Ae.albopictus was sensitivity to malathion,and the index is 1.05-1.91. Ae.albopictus from some cities also had developed resistance to other insecticides in different levels. Conclusion Resistance should be monitored regularly. Insecticides should be used scientifically and rationally to prevent and reduce the occurrence and development of resistance.

Objective To study the sensitivity of TaqMan MGB Real-time Polymerase Chain Reaction (PCR) on detecting Dengue virus (DV) from Aedes albopictus and to develop a sensitive,specific,and repetitive assay method for DV diagnosis. Methods Females A.albopictus were artificially infected with Den-2 in lab,different concentration for infecting mosquito (mosquitos/ 500 μl or mosquitoes/ 1 000 μl) and different pools (0,10,25,50 females of lab A.albopictus without DV were added to each treatment) were designed to process of virus detection by one-step and two-step TaqMan MGB Real-time PCR. Fluorescent signal was observed to show the results. Results For all pools,the minimal concentration which could be detected was 2 mosquitoes/ 500 μl and 3 mosquitoes/ 1 000 μl for two-step TaqMan MGB Real-time PCR,but 5 mosquitoes/ 500 μl for one-step TaqMan MGB Real-time PCR. There was no evident effect on the detection of DV RNA by the RNA of A.albopictus. Conclusion TaqMan MGB is sensitive and specific to detect DV,and is a perfect surveillance method for DV index of A.albopictus. It is better for 20-30 mosquitoes/pool and 500 μl dilution/pool by two-step TaqMan MGB Real-time PCR.

Objective To study the technology for Mus musculus Control. Methods To evoluate the acceptability of the bromadiolone poison paste and it's effect both in laboratory and in field. Results The Bromadiolone poison paste was stably performed in many conditions. It didn't get mouldy within 30 days. The intaking coefficient in laboratory was 1.56 . The consuming rate in field was 33.60% . The killing rate was 82.18% . Conclusion The bromadiolone poison paste tasted good to M.musculus,and can control M.musculus effective stably.